rabbit anti vegf a  (Bioss)

Journal: Cells

Article Title: Ovariectomy Induces Selective Alterations in Dura Mater Blood and Lymphatic Microvascular Network Architecture in Mice

doi: 10.3390/cells14211647

Figure Lengend Snippet: Increased α-SMA expression in CDM blood vessels and connective tissue stroma following OVX. ( A , B ) Representative immunofluorescence images of the CDM from sham-operated ( A ) and OVX ( B ) mice. VEGFR3-positive blood vessels are shown in red, α-SMA in green, and PDPL-positive lymphatic vessels in blue. ( C , D ) Quantitative analysis of α-SMA fluorescence intensity reveals a significant increase in α-SMA signal in blood vessels ( C ) and within CDM connective tissue stroma ( D ) in OVX mice compared to sham controls ( p = 0.04 and p = 0.009). Statistical significance was determined using an unpaired t -test. * p < 0.05, ** p < 0.01. Scale bar in ( B ): 100 µm.

Article Snippet: Blood vessels were identified using rabbit anti-VEGFR3 antibody (NSJ Bioreagents, San Diego, CA, USA; Cat. No. R32892 ; 1:100), followed by Alexa Fluor-conjugated goat anti-rabbit IgG (Thermo Fisher Scientific; Cat. No. A11072).

Techniques: Expressing, Immunofluorescence, Fluorescence

Journal: Journal of Nanobiotechnology

Article Title: Delivery of an ERK inhibitor using bioactive lipid nanoparticles reduces angiogenesis and prevents oral squamous cell carcinoma development

doi: 10.1186/s12951-025-03577-7

Figure Lengend Snippet: NPs-DPPA exert antiangiogenic effects through Angptl2. ( A ) Heatmap of the protein expression profiles of CAL-27 cells treated with NPs-DPPA. GO enrichment analysis ( B ) and volcano plot ( C ) of the differentially expressed proteins in CAL-27 cells treated with NPs-DPPA. Protein levels of ERK, P-ERK, VEGFa and Angptl2 in CAL-27 and SCC-7 cells ( D ) and a statistical analysis of the results (CAL-27 cells: E-H; SCC-7 cells: I-L) obtained from a western blot analysis after treatment with PBS, NPs-DPPA, ERKi, PLGA-ERKi, or NP-AE. ns, no significance; ** p < 0.01, *** p < 0.001, and **** p < 0.0001, n = 3; one-way ANOVA or two-tailed Student’s t test was used for multiple comparisons

Article Snippet: The VEGFA rabbit pAb (19003-1-AP), Angptl2 rabbit pAb (12316-1-AP), ERK rabbit pAb (11257-1-AP) and phospho-ERK rabbit pAb (28733-1-AP) were purchased from Proteintech (Wuhan, China).

Techniques: Expressing, Western Blot, Two Tailed Test